Reduced Background Expression and Improved Plasmid Stability with pET Vectors in BL21 (DE3)
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چکیده
منابع مشابه
Reduced background expression and improved plasmid stability with pET vectors in BL21 (DE3).
The T7 polymerase-based pET System is one of the most powerful and widely used prokaryotic expression systems available today. Expression of even slightly toxic gene products in BL21 (DE3), however, has been problematic due to basal expression, which leads to decreased plasmid stability and variable yields following large-scale growth and induction. Use of host strains such as BL21 (DE3) pLysS ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00296st03